LE23: Immunological Assays (GRADED ASSIGNMENT)

Before you begin the virtual assignment below, please read through the Background and Introduction sections of Lab Exercise 23: Immunological Assays.

Then, please complete all three of the protocols below:

Demonstration Protocol: ELISA

1. Watch the virtual ELISA protocol below.

Play media comment.

1. Record the results of the Indirect ELISA below in the Data Collection and Analysis section of your Lab Manual. NOTE: In the Discussion, it asks about the components of an indirect ELISA and then asks about patient Ag and lab Ab. Please correct these to patient Ab and lab Ag.

Before you begin the virtual experiments, please view this presentation

Virtual Experiment Protocol: Latex Agglutination Assay

1. Go to the URL: http://learn.chm.msu.edu/vibl/content/latexaglu.html. Links to an external site.
2. Open the module by clicking the hyperlink in the word module in Click to open the module.
3. Follow the protocol in your Lab Manual.
4. Record your data in the Data Collection and Analysis section of your Lab Manual.

Virtual Experiment Protocol: Lateral Flow Assay

1. Go to the URL: http://learn.chm.msu.edu/vibl/content/rapidstrep.html Links to an external site.
2. Open the module by clicking the hyperlink in the word module in Click to open the module.
3. Follow the protocol in your Lab Manual.
4. Record your data in the Data Collection and Analysis section of your Lab Manual.

Upload the Data Collection and Analysis & Discussion sections from Lab Exercise 23: Immunological Assays. 

NOTE: Canvas will only allow you to submit PDFs. There are many free apps that will allow you to take photos with your phone and convert the images to PDF. Please make sure you upload the entire assignment as a single PDF file.

I will grade these PDFs within Canvas and you will be able to see my feedback directly on them. To view your graded assignment do one of the following:

• From the Canvas app: Go to the assignment and click "Submission and Rubric."
• From the desktop: Go to the "Assignments" and click on the assignment name. On the right side of the page click on "Submission Details" and then click on "View Feedback" (located to the right of PDF link of your assignment).